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The specificity of a PCR reaction?

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Anu Smith:
Each and every step of PCR are very important. If any of the step is not performed properly or some mistake occurs then it is very difficult to get the result.

Kasim:
Brother Abdikasim is right...the annealing step is the most important one in PCR reaction.... this is because primers are very temperature specific and the amount of amplified product depends mainly on the efficiency of primers, their annealing temperature and time. then is the denaturation step.
Nice Discussion though

Muruganloyola:
As discussed by every one , In PCR most critical step is designing primer for specific target of expected genome or result, Primer designing includes various characteristics like, no primer dimer, primer should have more than 18 to 22 nucleotides, should rich in GC content, etc,


and another important step is optimising the exact annealing temperature of Primer with the target genome.

and optimising the reaction condition and the concentration of Primer in the reaction mixture are essential and important steps of PCR

I hope it would be useful for all..

thank you

ur

NMN

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